This package of MATLAB routines with accompanying GUI allows scientific users to measure the 3D location of single molecules when using the double-helix point spread function (DH-PSF) widefield microscope.
Features
- Double-Gaussian estimator is calibrated via an axial scan of bright immobile fluorescent emitters (e.g. beads).
- Template-matching and double-helix recognition algorithm is calibrated via setting an image correlation threshold against single-molecule data.
- Tiff stacks of SM images are analyzed using template matching followed by double-Gaussian fitting to extract estimates of the molecule positions.
- The motion/drift of fiduciaries (e.g. fluorescent beads) during an experiment can tracked and removed form SM localization data.
- 3D localization data can be exported to .csv format for post-processing and visualization.
- Basic 3D scatterplot and 2D z-projection histogram visualizations are included.
sdmixer
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Lab Storage
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